What are the Advantages of Yeast Peptones in the Fermentation Process?

Peptones are typically defined as partially hydrolyzed proteins that occur as liquids, pastes, powders, or granules. They can be obtained from animal, vegetal, and yeast proteins. While animal and vegetal peptones have been on the market for some time, yeast peptones are less known compared to other types of peptone available on the market. Yeast peptones offer numerous advantages not only in terms of being animal and allergen-free, but also in terms of batch consistency, biomass growth rate, and cell viability. Let’s have a look at what makes yeast peptones unique and the major benefits they can offer to the biotech industry.

Highlights

  • Yeast peptones offer a varied array of peptides and amino acids, which provide a rich nutrient source for cell and microbial growth.
  • Yeast peptones are a valuable addition to the peptone landscape, as they are animal and allergen-free.
  • Yeast peptones are compliant with food and biopharmaceuticals safety standards.
  • Yeast peptones are a cost-effective alternative to other peptones and provide a long-term visibility on supply.

Yeast peptones, a rich fermentation nutrient source

All peptones are composed of peptides and polypeptides resulting from the partial hydrolysis of peptide bonds present in raw material rich in proteins. In the case of yeast peptones, they are a protein hydrolysate derived from yeast (enzymatic hydrolysis). They are designed to contain a variety of small, medium, and long-chain peptides.

Yeast peptones offer a varied array of peptides and amino acids, which provide a rich nutrient source for cell growth. Its complex mixture closely mimics the nutritional complexity found in living organisms, making yeast peptones suitable for supporting a wide range of cell cultures.

Yeast peptones can allow producers to target more specific nutritional needs and achieve more specific outcomes in biotechnology processes such as:

  • Increased viability
  • Improved resistance to DSP
  • Optimal metabolite production

Yeast peptones serve as a vital nitrogen source suitable for the formulation of fit for purpose use culture media from the research and development scale to the industrial scale in many fields: biopharmaceuticals, recombinant protein, starter culture, probiotics, bioingredients, bulk fermentation and bio agriculture.

Yeast peptones are animal and allergen-free

One of the major advantages of yeast peptones over other forms of peptones is that they are both animal and allergen-free. Additionally, with yeast peptones, there is a low risk of contamination and as opposed to vegetal peptones production lines are guaranteed to be allergen-free. They meet the highest standard in quality and traceability.

Yeas peptones therefore are:

  • Animal-free, including lactose and dairy-free
  • Allergen-free, including gluten-free
  • Vegan
  • Cruelty-free

Yeast peptones support a variety of different microbes and cell types

There are over 1 million different bacterial species and each one of these microbes has its own nutritional needs. Yeast peptones can be tailored to the nutritional needs of various microbes that is being grown. Yeast peptones are a well-suited composition for promoting the growth of diverse cell types, such as fungi, bacteria, and mammalian cells. Because of their adaptability, yeast peptones are the go-to option for situations where a single peptone source needs to sustain a variety of cells or microbes.

This diversity is essential to cover the diversity of microbial nutritional needs in terms of:

  • Long chain peptides
  • Short chain peptides
  • Tripeptides
  • Dipeptides
  • Free amino acids

 

Yeast peptones are ideal for sustaining the growth, viability, and metabolic activity of lactic acid bacteria with complex nutritional requirements. They are also suitable for the industrial production of a broad range of Bifidobacteria.

Yeast peptones also have the benefit of being able to be used for the production of biopharmaceuticals such as biologicals (therapeutic proteins, vaccines, hormones such as insulin), active pharmaceutical ingredients (API), and vitamins by recombinant and non-recombinant microorganisms.

Yeast peptones ensure consistent quality

Yeast peptones that are sold commercially are manufactured under carefully regulated conditions, guaranteeing uniformity in both composition and quality. Because of this, they can be dependable parts of numerous biotechnological procedures that produce repeatable outcomes. This consistency is a major advantage, especially compared to other forms of peptones, which can become affected by external factors such as seasonality and adverse weather conditions. This is especially prevalent for peptones made from vegetable sources.

Furthermore, because yeast peptones are made through fermentation the process is highly standardized, which leads to lower batch-to-batch variability compared to other peptone sources.

Yeast peptones provide optimal cell growth

Yeast peptones enhance the diversity of microbial culture media by offering a variety of nutrients that promote the development of microorganisms. Moreover, the amino acid profile of yeast peptones is usually balanced, which is critical for the production of proteins and other cellular constituents. In applications involving bioprocessing and cell culture, this equilibrium may help promote the best possible cell growth and productivity.

Additionally, yeast peptones are designed to be rich in small-chain peptides and work synergistically with yeast extracts to ensure optimal cell growth for the industrial production of a broad range of mesophilic and thermophilic lactic bacteria. Yeast peptones also have the benefit of being able to optimize microbial cell viability.

Yeast peptones are a sustainable cost-effective alternative

Given the broad diversity of different types of peptones, there is also a wide range of prices in the peptone market. The price range for animal and vegetable peptones varies significantly from $15/Kg to $500/Kg. For highly specialized peptones prices can even reach $5,000/Kg.

Value of yeast peptones is high as they bring significant benefits in a process, whether it helps producers to fit consumers need or to reach higher productivity.

Furthermore, as the world moves towards a more carbon-neutral society, yeast peptones also offer a more sustainable form of peptones.

Yeast peptones are the future of peptones

While yeast peptones offer significant benefits the selection of a peptone is always contingent upon the particular needs of the intended use as well as the features of the cells or microbes being cultivated. Certain peptones, like meat or casein peptones, may be more suited for particular cell types and have different nutritional profiles, thus they might be favored in some circumstances. Nevertheless, yeast peptones do have significant advantages in many situations and ensure a high level of performance, which means they deliver:

  • Faster growth
  • Higher biomass
  • Higher yield
  • Balanced fermentation kinetics
  • Lower variability
  • Lower byproducts and waste
1) US Pharmacopeia (USP)